wheat germ agglutinin (Vector Laboratories)

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Vector Laboratories wheat germ agglutinin
Wheat Germ Agglutinin, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 884 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/L-1020/Unconjugated+Wheat+Germ+Agglutinin+(WGA)/pm42832292-50-3-9
Average 96 stars, based on 884 article reviews

wheat germ agglutinin - by Bioz Stars, 2026-10

96/100 stars

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Membrane:

Article Title: Alterations in cardiac function correlate with a disruption in fatty acid metabolism in a mouse model of SMA.
Article Snippet: Slides were blocked in 10% goat serum (for IB4) or 1% TritonX100 4% BSA (for Ki67) in PBS for 60 min before addition of primary antibody (biotinylated isolectin B4, I21414, Life Technologies, 1:100; Rabbit anti-Ki67 antibody, Abcam, Cat no ab15580, 1:200) overnight at 4◦C and visualised using secondary antibody (1:100, Streptavidin Alexa-488, S32354, Life Technologies; Donkey anti-rabbit IgG, Invitrogen ThermoFisher scientific, Cat no A-21206, 1:500). .. Rhodamine-conjugated wheat germ agglutinin was applied where required to label the cell membrane (1:75, RL1022, Vector Laboratories). .. Slides were exposed to 4′, 6-diamidino2-phenylindole (DAPI; Life Technologies; 1:1000) and mounted in MOWIOL mounting media (Sigma).

Whole Genome Amplification:

Article Title: A Redox-Switchable Supramolecular Carbohydrate Chip for Capture and Real-Time Glycosylation Analysis of Living Suspension Cells
Article Snippet: Dopamine hydrochloride, concanavalin A (Con A), D-galactose, tris(hydroxymethyl)aminomethane (Tris) were purchased from Sigma-Aldrich (St. Louis, MO, USA). .. Wheat germ agglutinin (WGA), Ulex europaeus agglutinin I (UEA-I), and soybean agglutinin (SBA) were purchased from Vector Labs (Burlingame, CA, USA). .. Hexanediamine, Nacetylglucosamine (GlcNAc), D-mannose, and L-fucose were obtained from Sarn Chemical Technology (Shanghai, China).

Article Title: A genome-wide atlas of human cell morphology.
Article Snippet: After RCA, the cells were prepared for phenotypic labeling by incubating them with a blocking buffer containing 1% BSA (Seracare Life Sciences, 1900-0016) in 1× PBS for 10 min at room temperature. .. After blocking, a primary staining solution containing rabbit anti-TOMM20 Nature Methods Resource https://doi.org/10.1038/s41592-024-02537-7 antibody (Abcam, ab78547), Alexa Fluor 488 Phalloidin (Thermo Fisher, A12379), ConA-SS-A647 and WGA-A750 (WGA protein by Vector Labs, L-1020-20, custom conjugation to A750 fluorophore by Arvys Proteins) was prepared in 1× PBS and applied to the cells for 45 min at room temperature. .. After blocking, a primary staining solution containing rabbit anti-TOMM20 Nature Methods Resource https://doi.org/10.1038/s41592-024-02537-7 antibody (Abcam, ab78547), Alexa Fluor 488 Phalloidin (Thermo Fisher, A12379), ConA-SS-A647 and WGA-A750 (WGA protein by Vector Labs, L-1020-20, custom conjugation to A750 fluorophore by Arvys Proteins) was prepared in 1× PBS and applied to the cells for 45 min at room temperature.

Blocking Assay:

Staining:

Conjugation Assay:

Saline:

Article Title: The Nucleus of the Brachium of the Inferior Colliculus Contains Thalamic-Like and Midbrain-Like Subregions.
Article Snippet: .. C reat .5% wheat germ agglutinin (WGA; L ‐1020, Vector Laboratories, ewark, CA, USA) or 0.2%–2% fluorogold (FG; #80014, Biotium, remont, CA, USA) dissolved in saline was placed on the pial urface for 10 min ..

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